The latest arrests bring to five the number of people in Police custody over the murder.
Police at Akosombo exhumed the bodies of the Americans a day after they were reported missing from their home in Akwamu.
The District Security Council has been meeting over the incident. Meanwhile, the Embassy of the United States of America in Ghana has expressed regret over the killing of its two citizens.
The Embassy in a statement Thursday said it is already in contact with the families of the deceased and will provide the necessary consular assistance to them.
“The U.S. Embassy in Ghana reports with deepest sympathy that two U.S. citizens have been killed in the Eastern Region of Ghana.
“We express our deepest condolences to the family and friends of the victims. The U.S. Embassy in Ghana is in contact with family members and is providing appropriate consular assistance. Due to privacy considerations, we have no further comment at this time.
“We have confidence in the rule of law in Ghana, and urge any residents with information regarding this incident to report it to the Ghana Police Service. We refer you to local authorities for details on the investigation,” the statement said.
The Police in Akosombo exhumed the bodies of the two Americans on Wednesday, hours after they had been killed and buried.
A search for them started after a puddle of blood and a blood-stained cudgel, were found in their home Tuesday.
The deceased, 75-year-old woman identified as Mamlena and 60-year-old Nzinga were leaders of Fiankra, a symbolic African American community in Akwamu. They were, at a point in time, according to their Lawyer Samson Lardy Anyenini, accused by chieftaincy Minister, Dr. Seidu Danaa, of illegally acting as chiefs.
Mr Anyenini Thursday told Starr FM that the Americans had been living in fear after a Ghanaian known as Osofo–who played a role in getting the National House of Chiefs establish the Fiankra stool and skin for the diasporans–seized one of their houses.
During the animosity, a dog belonging to the Americans was allegedly poisoned.
The case was withdrawn from the court and settled amicably following an intervention by the Attorney General.